双光子成像

(1)仪器简介:

型号:Olympus FV1200MPE

仪器的主要性能指标:激光器:488/633/405三个波长激光器;分析参数:12色荧光、前向散射光、侧向散射光;分析速度:>100,000个/秒。

仪器用途:对厚标本和活细胞或活体动物等进行观察分析,可实现对动物组织如脑的活体成像。荧光定量定性分析,荧光原位杂交分析,蛋白亚细胞定位分析,蛋白相互作用,3D成像技术,时间序列成像等。。

(2)实验方案及收费标准

(3)案例:

A.人外周血调节性 T细胞亚群表型分析:

Xin L, Gao J, Ge X, Tian C, Ma W, Tian Z, Zheng X, Hou J. Increased pro-inflammatory cytokine-secreting regulatory T cells are correlated with the plasticity of T helper cell differentiation and reflect disease status in asthma. Respir Med. 2018 Oct;143:129-138.

B.小鼠记忆Tfh细胞表性分析:

Duan X, Sun P, Lan Y, Shen C, Zhang X, Hou S, Chen J, Ma B, Xia Y, Su C. 1IFN-α Modulates Memory Tfh Cells and Memory B Cells in Mice, Following Recombinant FMDV Adenoviral Challenge. Front Immunol. 2020 Apr 29;11:701.